Modifying Enzymes
Aldevron Eureca-V Nuclease, 0.25mg
0.25mg dispense of Eureca-V Nuclease. CRISPR protein, Eureca-V™ Nuclease is the most recent addition to our catalog. It is based on the proprietary MAD7 nuclease developed by Inscripta. Aldevron and Inscripta have partnered to make this protein broadly available to the therapeutic, diagnostic, and agricultural research markets.Eureca-V is categorized as a Class 2, Type V CRISPR-Cas nuclease. It shares key features common to this family of CRISPR nucleases, including:
•Targets broad array of T-rich PAM domains (YTTV preferred, TTTN functions)
•Requires a single crRNA to cut target DNA sequence
•Staggered double-strand break at cut site creates 5’ overhangs
This product is subject to the following: Limited Use Label License
Aldevron SpCas9 Nuclease, 5mg
5mg dispense of SpCas9 Nuclease. Aldevron provides Research-grade Cas9 nucleases via Fisher Scientific for use in development work as well as standard cGMP products for clinical studies when ordered direct.
Functional Performance
•Ready to use for electroporation or transfection
•Extensive QC panels
•10 mg/mL formulation
LGC GENOMICS LLC (Lucigen) Ampligase DNA Ligase without Buffer, 10,000 U @ 100 U/LHighly thermostable DNA ligase for applications where ligation at high temperature is beneficial or required.
Ampligase Thermostable DNA Ligase catalyzes NAD-dependent ligation of adjacent 3-hydroxylated and 5-phosphorylated termini in duplex DNA structures that are stable at high temperaturesHigh thermostability allows ligation using high-stringency hybridization conditionsHigh specificity and stringency permits sensitive detection of SNPsAvailable in kit and "enzyme only" formats11
Sigma Aldrich Fine Chemicals Biosciences Lysostaphin from Staphylococcus staphylolyticus lyophilized powder, Protein 50-70 % by biuret, >=500 units/mg protein | 9011-93-2 | MFCD00131556 | 1MG
Lysostaphin from Staphylococcus staphylolyticus lyophilized powder, Protein 50-70 % by biuret, >=500 units/mg protein | 9011-93-2 | MFCD00131556 | 1MG
Agilent Technologies GLYKO SIALIDASE ASAMPLER KIT
Try our lower MW isoforms for challenging sialidase digests.This Sampler Kit contains 1 vial each of 3 isoforms (88, 66 and 51 kDa) of the enzyme Sialidase A. Lower MW sialidases may help desialylate glycans if steric hindrance is an issue with regular sialidase.
INTACT GENOMICS INC Endonuclease IV (Nfo) 5000 Units (10 units/µl)
Endonuclease IV (Nfo) from Escherichia coli is a 32-kD metalloprotein that aids in the repair of damaged DNA. The enzyme functions both as an apurinic/apyrimidinic nuclease (1) and as a 3′-terminal di-esterase (1-4). Its 3′-terminal di-esterase activity is important in the repair of DNA strand breaks generated by oxidation and ionic radiation (2, 3). In such events, the strand breaks terminate with either a 3′ phosphate or a deoxyribose fragment, preventing repair by DNA polymerase I or DNA ligase. Endonuclease IV removes the blocking groups, leaving a free 3′-hydroxyl terminus. This enzyme does not have detectable associated exonuclease or DNA N-glycosylase activity (1).Applications• Single cell gel electrophoresis (Comet assay) (5, 6)• Alkaline elution (7)• Alkaline unwinding (8)Product SourceE. coli BL21 (DE3) strain expressing E. coli Endonuclease IV gene.Product Includes• Endonuclease IV (Nfo)• 10x Endonuclease IV reaction buffer
Medchemexpress LLC Catalase, aspergillus niger | 9001-05-2 | 500 MG
Catalase, Aspergillus niger is a key enzyme involved in the metabolism of H2O2 and reactive oxygen species (ROS), with its expression and localization being significantly altered in tumors. It functions as a free oxygen radical scavenger.
- Produced through fermentation by Aspergillus niger.
- Effective within a pH range of 4.0 to 10.0.
- Effective within a temperature range of 35°C to 40°C.
- Can be dissolved using water or as specified in relevant references.
- The active site contains an iron atom.
- Contains a N-terminal arm with a distal histidine, essential for the catalase reaction.
- Features a β-barrel domain with eight β-barrels arranged antiparallel with six α-helical insertions, forming the hydrophobic core.
- Includes a connection domain containing a tyrosine residue that binds the heme group.
- Has α-helical domains important for NADPH binding.
Carnabio Usa Inc ROCK1/10UG/CATALYTIC
ROCK1, Catalytic domain, Wild type, Amino Acid 1-477, NP_005397.1, Expressed in Insect (sf21); 10microg
LGC GENOMICS LLC (Lucigen) Terminator 5'-Phosphate-Dependent Exonuclease, 40U @ 1 U/LA processive 5 to 3 riboexonuclease that specifically digests RNA with 5-monophosphate ends only.
Terminator™ 5-Phosphate-Dependent Exonuclease is a processive 53 exonuclease that digests RNA that has a 5-monophosphate end. It does not digest RNA that has a 5-triphosphate, 5-cap or 5-hydroxyl group.
Research Products International Corp Ribonuclease A, Bovine Pancreas, 250 Milligrams
Molecular Biology Grade. Suitable for use in removing RNA in nucleic acid applications and where other enzymes are used or intact proteins need to be recovered. Chromatographically purified and supplied as a lyophilized powder.
Activity: ≥2,500 units/mg (dry weight)
Sigma Aldrich Fine Chemicals Biosciences Proteinase from Aspergillus melleus Type XXIII, >=3 units/mg solid | 9001-92-7 | MFCD00132092 | 25G
Proteinase from Aspergillus melleus Type XXIII, >=3 units/mg solid | 9001-92-7 | MFCD00132092 | 25G
Sigma Aldrich Fine Chemicals Biosciences Proteinase K from Tritirachium Album | 39450-01-6 | MFCD00132129 | 1ml
Proteinase K from Tritirachium Album | 39450-01-6 | MFCD00132129 | 1ml
Sigma Aldrich Fine Chemicals Biosciences Phosphatase, Acid from potato lyophilized powder, >=3.0 units/mg solid | 9001-77-8 | MFCD00131847 | 50UN
Phosphatase, Acid from potato lyophilized powder, >=3.0 units/mg solid | 9001-77-8 | MFCD00131847 | 50UN